Karyotype and Sex Expression in Coccinia indica, a Dioecious Cucurbit
نویسندگان
چکیده
Cytological and immunochemical studies were carried out on dioecious Coccinia indica to understand the genetic control of sex expression. The somatic chromosome number of both the sex forms of C. indica was found to be 2n = 24 and the karyotype shows high homogeneity, though a distinct heteromorphic pair of sex chromosomes is found in male sex forms of C. Indica. Soluble protein profile from the tuberous roots of the male and female plants of C. indica did not show any marked distinction, only a variation in the staining pattern was observed. However, in the immunoblot assay the cross reactivity of anti-p-22 (antibody raised against a female biased 22 kD protein of Momordica dioica) at 29 kD and 32 kD regions of the protein profiles in both sex forms establishes p-22 as a sex linked marker. The ratio of the intensity of 29/32 kD polypeptide is also an indicator of sex expression and the data has been analysed as well.
منابع مشابه
Cucurbitane-Type Triterpenoids from the Blood Glucose-Lowering Extracts of Coccinia indica and Momordica balsamina Fruits
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